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Regulatory

Part:BBa_K818100:Design

Designed by: Emeraldo Jo   Group: iGEM12_Groningen   (2012-09-19)

Promoter sboA, upregulated by rotten meat volatiles in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We identified the PsboA promoter and characterized the working in Bacillus subtilis.

References

Nakano M. M., Zheng G., Zuber P. (2000). Dual control of sboa-alb operon expression by the Spo0 and ResDE systems of signal transduction under anaerobic conditions in Bacillus subtilis. Journal of Bacteriology, 181(11): 3274-3277

Source

This part is amplified from B. subtilis 168 genome.

References